منابع مشابه
The Immunochemistry of Toxins and Toxoids
Methods for the purification and crystallization of tetanal toxin are described. The methods consist of the multiphase fractionation system involving methanol as the organic precipitating agent under controlled conditions of pH, ionic strength, protein concentration, and temperature. Crystalline tetanal toxin has an electrophoretic mobility of 2.8 x 10(-5) in veronal buffer of 0.1 ionic strengt...
متن کاملMass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids
Bordetella pertussis (Bp) is the causative agent of pertussis, a vaccine preventable disease occurring primarily in children. In recent years, there has been increased reporting of pertussis. Current pertussis vaccines are acellular and consist of Bp proteins including the major virulence factor pertussis toxin (Ptx), a 5-subunit exotoxin. Variation in Ptx subunit amino acid (AA) sequence could...
متن کاملThe Immunochemistry of Toxins and Toxoids v. the Solubility of Staphylococcal Toxin in Methanol- Water Mixtures under Controlled Conditions of Ph, Ionic Strength, and Temperature*
The success encountered in the purification of tetanal toxin (1) and toxoid (2, 3), of diphtherial toxoid (4), and of soluble pertussal antigens (5) warranted attempts to purify other toxins and toxoids by the multiphase system of protein fractionation. The system employs the precise adjustment of methanol concentration, pH, ionic strength, and protein concentration under rigidly controlled con...
متن کاملThe immunochemistry of toxins and toxoids; the solubility of staphylococcal toxin in methanol-water mixtures under controlled conditions of pH, ionic strength, and temperature.
The success encountered in the purification of tetanal toxin (1) and toxoid (2, 3), of diphtherial toxoid (4), and of soluble pertussal antigens (5) warranted attempts to purify other toxins and toxoids by the multiphase system of protein fractionation. The system employs the precise adjustment of methanol concentration, pH, ionic strength, and protein concentration under rigidly controlled con...
متن کاملthe analysis of the role of the speech acts theory in translating and dubbing hollywood films
از محوری ترین اثراتی که یک فیلم سینمایی ایجاد می کند دیالوگ هایی است که هنرپیش گان فیلم میگویند. به زعم یک فیلم ساز, یک شیوه متأثر نمودن مخاطب از اثر منظوره نیروی گفتارهای گوینده, مثل نیروی عاطفی, ترس آور, غم انگیز, هیجان انگیز و غیره, است. این مطالعه به بررسی این مسأله مبادرت کرده است که آیا نیروی فراگفتاری هنرپیش گان به مثابه ی اعمال گفتاری در پنج فیلم هالیوودی در نسخه های دوبله شده باز تولید...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30839-6